350 centrifugal buffer exchange column Search Results


trk lysis buffer  (Omega Bio Tek)
90
Omega Bio Tek trk lysis buffer
Trk Lysis Buffer, supplied by Omega Bio Tek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/trk+lysis+buffer/pm37238698-82-11-14
Average 90 stars, based on 1 article reviews
trk lysis buffer - by Bioz Stars, 2026-10
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dna rna shield solution  (Zymo Research)
99
Zymo Research dna rna shield solution
Dna Rna Shield Solution, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/DNA%2FRNA+Shield/10__1002_slash_edn3__596-63-27-30
Average 99 stars, based on 1 article reviews
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lysis buffer  (Zymo Research)
95
Zymo Research lysis buffer
Lysis Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/Solution+2+Lysis+Buffer/pm29939785-70-10-15
Average 95 stars, based on 1 article reviews
lysis buffer - by Bioz Stars, 2026-10
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tris buffered saline  (Bio-Rad)
98
Bio-Rad tris buffered saline
Tris Buffered Saline, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/Tris/pmc09561518-61-45-30
Average 98 stars, based on 1 article reviews
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xbal 1  (New England Biolabs)
99
New England Biolabs xbal 1
Xbal 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/NEBuffer+2/pm16306208-66-17-19
Average 99 stars, based on 1 article reviews
xbal 1 - by Bioz Stars, 2026-10
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renilla luciferase reagent  (NanoLight Inc)
90
NanoLight Inc renilla luciferase reagent
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Renilla Luciferase Reagent, supplied by NanoLight Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/renilla+luciferase+substrate+coelenterazine+h/pmc06575207-177-51-59
Average 90 stars, based on 1 article reviews
renilla luciferase reagent - by Bioz Stars, 2026-10
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lysis buffer  (Promega)
90
Promega lysis buffer
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/lysis+buffer/pmc09819203-113-35-37
Average 90 stars, based on 1 article reviews
lysis buffer - by Bioz Stars, 2026-10
90/100 stars
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balanced salt solution  (Thermo Fisher)
99
Thermo Fisher balanced salt solution
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Balanced Salt Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/Phosphate/pm28655854-210-26-74
Average 99 stars, based on 1 article reviews
balanced salt solution - by Bioz Stars, 2026-10
99/100 stars
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buffer  (Bio-Rad)
96
Bio-Rad buffer
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/Bromophenol+Blue/pm38339099-330-10-60
Average 96 stars, based on 1 article reviews
buffer - by Bioz Stars, 2026-10
96/100 stars
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pbs rinse  (Thermo Fisher)
99
Thermo Fisher pbs rinse
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Pbs Rinse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/PBS/10__1016_slash_j__bmc__2018__04__009-154-38-47
Average 99 stars, based on 1 article reviews
pbs rinse - by Bioz Stars, 2026-10
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avs 350 viscometer  (SCHOTT)
90
SCHOTT avs 350 viscometer
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Avs 350 Viscometer, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/350+centrifugal+buffer+exchange+column/ubbelohde+viscometer/10__1021_slash_acs__iecr__3c02684-59-24-24
Average 90 stars, based on 1 article reviews
avs 350 viscometer - by Bioz Stars, 2026-10
90/100 stars
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detectx corticosterone elisa kit k014-h5  (Arbor Assays)
90
Arbor Assays detectx corticosterone elisa kit k014-h5
HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well <t>ISRE-luciferase</t> reporter plasmid and 10 ng per well <t>Renilla</t> luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Detectx Corticosterone Elisa Kit K014 H5, supplied by Arbor Assays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well ISRE-luciferase reporter plasmid and 10 ng per well Renilla luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Histone deacetylase 4 promotes type I interferon signaling, restricts DNA viruses, and is degraded via vaccinia virus protein C6

doi: 10.1073/pnas.1816399116

Figure Lengend Snippet: HDAC inhibitors TSA and LMK235 (LMK) inhibit the response to IFN-α. ( A ) HeLa cells seeded in 96-well plates were cotransfected in triplicate with 100 ng per well ISRE-luciferase reporter plasmid and 10 ng per well Renilla luciferase plasmid overnight. Cells were then treated simultaneously with 250 nM TSA and 1,000 units/mL IFN-α for 6 h. After cytokine stimulation, a cell lysate was harvested and firefly luciferase was measured and normalized to Renilla luciferase control. The fold induction of firefly luciferase is shown relative to unstimulated controls. ( B and C ) LMK235 inhibits the response to IFN-α but not IL-1β. HeLa cells were transfected as in A except in C where an NF-κB-luciferase reporter was used. Cells were then treated simultaneously with the indicated concentrations of LMK235 and 1,000 units/mL IFN-α or 100 μg/μL IL-1β for 6 h. The fold increase in firefly luciferase expression, normalized to Renilla control, is presented as in A . ( D – G ) LMK235 inhibits ISG expression in response to IFN-α. HeLa cells were treated simultaneously with 1 μM LMK235 or DMSO and 1,000 units/mL IFN-α ( D – F ) or 100 ng/mL IL-1β ( G ) for 6 h. mRNA was extracted from cells and used for RT-qPCR analysis. Data are presented as the fold induction of mRNA expression relative to the unstimulated, DMSO-treated control and relative to GAPDH mRNA expression. Data shown are representative of three independent experiments. ns = not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: To measure the luciferase activity, 50 μL firefly luciferase reagent [20 mM tricine, 2.67 mM MgSO 4 ⋅7H 2 O, 0.1 mM EDTA, 33.3 mM DTT, 530 μM ATP, 270 μM acetyl-CoA, 132 μg/mL luciferin (Prolume), 5 mM NaOH, 0.26 mM MgCO 3 Mg(OH) 2 ⋅5H 2 O], or 50 μL Renilla luciferase reagent (2 μg/mL coelenterazine in PBS) (Nanolight Technology, 350-10) was added to 10 μL cell lysate.

Techniques: Luciferase, Plasmid Preparation, Control, Transfection, Expressing, Quantitative RT-PCR